The SmartLid Blood DNA/RNA Extraction Kit has been designed for the rapid extraction of DNA and RNA from whole blood. Uniquely, the kit is capable of lysing and purifying nucleic acids from viruses, gram-negative bacteria, gram-positive bacteria, and even certain parasites, with all required buffers and enzymes included.
Features
Rapid
SmartLid is based on a proprietary magnetic key and lid, designed to quickly and easily transfer magnetic beads and attached nucleic acids through a series of three simple sample extraction steps: Lysis, Wash, and Elution.
High Yield
Ultra-pure DNA/RNA for sensitive downstream applications in molecular biology.
Broad Compatibility
Efficiently extract nucleic acids from viruses, bacteria (both gram-negative and gram-positive), and certain parasites
Easy to use
Simple process does not require any centrifugation and minimizes pipetting
Accessories
SmartLid Rack and Vortex Tool enable simultaneous processing of up to 12 samples
Environment
Designed to minimise plastic waste.
Carton and paper inserts made with recycled content and are fully recyclable.
Magnetic keys are reusable, reducing waste.
Specifications
Applications:
PCR, RT-PCR, qPCR, RT-qPCR, LAMP, RT-LAMP, dPCR, RT-dPCR (and other amplification chemistries)
Technology:
Superparamagnetic beads (silica)
Main Sample Types:
Whole Blood (also compatible with plasma and serum)
Isolated Molecules:
DNA, RNA
Sample Input Volume:
100 μL recommended (up to 200 μL possible)
Elution Volume:
50 μL recommended
A260/A280 Ratio
1.7-2.0 (typical range)
Protocol time:
<20 minutes (viruses and gram-negative bacteria), <45 minutes (gram-positive bacteria)
Storage conditions:
Room temperature (15-25°C)
Limitations:
Not intended for the diagnosis, prevention, or treatment of a disease
How it Works
SmartLid is based on a novel (patent pending) magnetic key and lid, designed to quickly and easily transfer magnetic beads and attached nucleic acids through a series of three simple sample extraction steps: Lysis, Wash, and Elution.
Visible Efficiency
The visible speed and efficiency of SmartLid is due to superparamagnetic beads, delivering ultra-fast extractions.
Capture: Magnetic beads capture DNA/RNA for transfer between tubes.
Collect: Inverting the tube with the
Magnetic Key collects the magnetic
beads onto the SmartLid.
Transfer: Within seconds the liquid is clear, and collection is complete and ready to transfer.
Extraction Workflow
Using SmartLid is an easy, simple process that requires no electricity, minimal pipetting, and only three simple steps, enabling market leading ease-of-use.